Expression of a Self-Incompatibility Glycoprotein (S2-Ribonuclease) from Nicotiana alata in Transgenic Nicotiana tabacum.
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منابع مشابه
Expression of a Self-Incompatibility Glycoprotein (S2-Ribonuclease) from Nicotiana alata in Transgenic Nicotiana tabacum.
In Nicotiana alata, self-incompatibility is controlled by a single locus, designated the S-locus, with multiple alleles. Stylar products of these alleles are ribonucleases that are secreted mainly in the transmitting tract tissues. N. tabacum plants were transformed with constructs containing the S2-cDNA and genomic S2-sequences from N. alata that were linked to the cauliflower mosaic virus 35S...
متن کاملIdentification, isolation, and N-terminal sequencing of style glycoproteins associated with self-incompatibility in Nicotiana alata.
S-Gene-associated glycoproteins (S-glycoproteins) from styles of Nicotiana alata, identified by non-equilibrium two-dimensional electrophoresis, were purified by cation exchange fast protein liquid chromatography with yields of 0.5 to 8 micrograms of protein per style, depending on the S-genotype of the plant. The method relies on the highly basic nature of the S-glycoproteins. The elution prof...
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Three alleles of the self-incompatibility gene of Nicotiana alata have been cloned and sequenced. A comparison of the sequences shows a surprisingly low level of homology (56%) and the presence of defined regions of homology and variability. The homologous regions include the N-terminal sequence, most of the cysteine residues and glycosylation sites, as well as other blocks throughout the seque...
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The use of tobacco dates back to the ancient civilizations of the Americas, where it played a central role in religious occasions. The peoples smoked tobacco in cigars and pipes and chewed it with lime, for its pleasurable euphoriant effects. In the 16 th century, Europeans spread the use of tobacco in North America, while the Spanish bring it into Europe. In the 1559, Jean Nicot, the French am...
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Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 µg 14D9ml-1. intracellular 14D9 amounts with a yield increase up to 20.82 µg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 µg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the cul...
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ژورنال
عنوان ژورنال: The Plant Cell
سال: 1992
ISSN: 1040-4651,1532-298X
DOI: 10.1105/tpc.4.9.1063